* In any HotSpringsEpisode, when the women have discovered the fact that they are being peeped on, they will do this in unison.
* Pedro from ''Anime/ExcelSaga'' is infamous for doing the Big No at least {{Once an Episode}}.
* ''Anime/MazingerZ'': Kouji Kabuto, right after his grandfather dies on his arms: "GRANDPA! NOOOOOOOOOOO!!!"
** ''Anime/GreatMazinger'': Tetsuya, after realizing [[spoiler:his adoptive father had pulled out a kamikaze attack to save his life]]: "NOOOOOOOOOOOOOOOOOOO!!!!!!!"
** ''Anime/UFORoboGrendizer'': Duke Fleed after watching [[spoiler:several hundred of children being murdered by the Vegans]]: "NOOOOOOOOOOOOO!!!!!"
* Used straight in ''RecordOfLodossWar'' by two characters at the same time.
* Used in ''TrinityBlood'' at the climax of episode 22.
* ''SonicX'':
** Used at the climax of episode 3 by Amy Rose, Sonic's self-appointed "girlfriend".
** Shadow the Hedgehog does this in episode 61 while trying to defeat the Metarex ship.
* The English dub of ''VisualNovel/{{Kanon}}''(2006) has the young Yuichi screaming "NOOOOOOOOOO!" after [[spoiler: Ayu falls off a tree and apparently dies (she actually slips into a [[ConvenientComa coma]]). In the Japanese version Yuichi instead screams "[[SayMyName AYUUUUUUUUUU]]!"]]
* Though the series engages in more and more towards the end, the biggest of Big Noes in ''Anime/NeonGenesisEvangelion'' are one by Asuka [[spoiler:at the end of [[http://www.youtube.com/watch?v=VJ0doO3VFjc her]] [[http://www.youtube.com/watch?v=cM-OOy9jc04 Mind Rape]]]], one by Shinji after [[spoiler: realizing his Mecha has been used by his father to crush the Entry Plug loaded with his unconscious best friend]], and one by Maya Ibuki (who spends most of ''End of Evangelion'' freaking out) after [[spoiler:[[FanNickname Giant Naked Rei]]'s hand passes right through her entire body]]. Some of these were dubbed into English as inarticulate yells, probably to avoid being straight-up {{Narm}}.
** Since the Japanese word usually used for the Big No, "iya," sounds like a scream anyway, it only makes sense to translate these as such.
* Used in ''{{Nadia The Secret of Blue Water}}'' by the title character, twice in the final episode [[spoiler:first when Emperor Neo sacrifices his life to free her from Gargoyle's control, and, even more heartbreakingly, when Gargoyle kills Jean by making him fall to the floor. (Nadia eventually revives him with the Blue Water.)]]
* In ''Anime/DigimonAdventure'', [[spoiler:Hikari does one when Wizardmon dies, leading to the activation of her crest]]. It was strangely cut from the dub. Equally as strange, the clip was shown in a brief flashback in a later episode, except during a voiceover narration, so her voice was not heard.
** Also used in the episode "Ogremon's Honor" by Mimi when [[ItWasHisSled Leomon dies]]
* ''LiarGame'' plays this straight with Ms. Kosaka upon her [[http://www.onemanga.com/Liar_Game/82/15/ defeat]].
* ''Anime/{{Mai-HiME}}'' has more than a few instances of this happening. Of course, what with all that's going on in that series (especially later on), the characters can be forgiven for yelling {{Big No}}s.
* ''MagicalGirlLyricalNanoha''
** IllGirl Hayate has a huge, primal one near the end of the second season. Considering how it punctuates a particularly nasty BreakTheCutie moment, the overall effect is quite chilling.
** In the fourth Megami sound stage, Shamal gives one in a more light-hearted moment when [[spoiler:Agito]] inadvertently shows Hayate a cake from Nanoha's parents' cafe that was supposed to be a surprise (as part of celebrating the tenth anniversary of Hayate recovering) due to being unable to read the kanji on the box.
** Fate has one in the one-shot "[[HasTwoMommies Second Mother's Day]]," when she worries that when Vivio doesn't give her a gift for Mother's day, she will soon stop seeing her as a mother and eventually no longer recognize her.
** [[SmugSnake Quattro]] gets one of these off just seconds before [[MamaBear Nanoha's]] DungeonBypass WaveMotionGun [[CrowningMomentOfAwesome utterly frags her]].
* In a mid-series episode of ''Anime/MobileSuitGundamWing'', Duo gives one out when Trowa destroys his Gundam[[note]]As part of an IfYoureSoEvilEatThisKitten situation[[/note]]. It's even bigger in the US dub; voice actor Creator/ScottMcNeil has said in order to achieve this, he thought about the time his wife ran over his motorcycle with the family minivan. It's actually so famous that he's often asked to re-enact it at conventions.
* In ''{{Robotech}} The Shadow Chronicles'' there are two moments - first when [[spoiler: Commander Tailor dies]] and then when [[spoiler: Alex Romero sacrifices himself. This one is especially strong.]]
* Simon from ''TengenToppaGurrenLagann'' performs a variation on this trope, instead shouting "[[spoiler:ANIKI!!!]]" when he believes [[spoiler: Kamina has been killed by Thymilph. Kamina, however, stays alive just long enough to make a HeroicSacrifice in the form of the Giga Drill Breaker]].
** The king (or rather, queen) of this trope is - surprise, surprise - Kinon. [[spoiler:When Rossiu is about to shoot himself in the head, [[LoveInterest Kinon]] screams out a teary '''''DAAMEEEEEEEEEEE!!!''''' which stops him dead in his tracks - just in time to receive a GetAHoldOfYourselfMan punch from Simon.]]
* ''Manga/FullmetalAlchemist''
** [[spoiler: The second]] Greed gets one of these [[spoiler: after the revived and amnesiac Greed unknowingly kills the last living member of the former Greed's QuirkyMinibossSquad]]. Ling, [[spoiler:whose body the current Greed is inhabiting,]] asks [[WhatTheHellHero What The Hell Anti-Villain]], causing [[spoiler:the old Greed's memories to resurface, with the new one]] letting out a screen-filling, gut-wrenching, Big No.
** Hohenheim [[spoiler:pulls an inarticulate scream of despair and horror]] after [[spoiler:he realizes he's the LastOfHisKind (the last human alive in Xerxes, his country, to be exact) and has become a living philosopher's stone]].
** In chapter 107, [[spoiler: Ed lets out a Big No when he realizes Al is sacrificing his own soul to restore Ed's real arm and save him from Father.]]
** Episode 45 of ''Brotherhood'' sees LargeHam Alex Armstrong giving big no after big no. He's fighting his sister, FourStarBadass Oliver Armstrong.
* ''Manga/{{Bleach}}''
** Kon does one in episode 15.
** In anime episode 79 (the Bount arc), Uryu Ishida has one after Yoshino is killed.
** Shinji does this in the flashback (Turn Back the Pendulum) arc.
** In anime episode 217, Charlotte Cuuhlhourne does one just before he's killed by Yumichika's sword.
** In Chapter 349, [[spoiler:Orihime]] gets one that spans '''two pages''' after [[spoiler:Ulquiorra uses his Cero to blow a hole in Ichigo's chest]]. Not only that, she even needs to take a breath before continuing with it.
** Toshiro Hitsugaya in chapter 392 when he realizes he's stabbed [[spoiler:Momo and not Aizen in the chest.]]
** In manga chapter 459, [[spoiler:Ichigo Kurosaki when Kūgo Ginjō steals his Fullbring.]]
** An arrancar girl does this when [[PsychoLesbian Chizuru]] makes perverted advances towards her in the anime.
* ''Anime/SailorMoon''. A lot, but particularly in the last episodes of the first and last seasons when all her friends [[spoiler:seemingly die]].
* In ''CodeGeass'', Lelouch has one when he loses a life-or-death game of chess to [[PsychopathicManchild Mao]] -- and the life on the line was [[BigBrotherInstinct Nunnally]]. [[spoiler:Though as it turns out, she was perfectly safe and he [[MagicalEye Geassed]] himself for some EnforcedMethodActing.]]
** The English dub presents us with Suzaku's epic [[http://www.youtube.com/watch?v=gJX-YTPZVH4 (g)NOOOOOOOOO(me)!]]
* ''FushigiYuugi'' makes use of this trope through Miaka, Tamahome, Miaka, Chichiri, Miaka, Yui, Miaka...
* ''DragonBallZ''
** Super Buu's Big No [[spoiler:rips a hole in a dimension.]]
** Earlier in the same season (in the original and English dub), Bulma let out one upon learning that [[spoiler:Vegeta had been killed by Buu]].
** Even earlier, in the dub only (he screams "Goku" in the original), Krillin uses one when it becomes clear that [[spoiler:Goku is going to sacrifice himself to take Cell away]].
** In the dub, Trunks seemed to have mastered the Narm of the Big No, with lines like "If they let that android free, it will be the end of all of us! NOOOOOOOOOOOOOO!" (Lampshaded in this fanvid: http://www.youtube.com/watch?v=_HOAETv_nVo) and not too long after "FATHER! hurgh, NOOOOOO! FATH-ER!"
** But Trunks also has a decidedly non-narmy Big No in the dub of his OVA when he finds Future Gohan dead. In the original Japanese, it's a simple SkywardScream.
** Earlier than the other times, Goku lets out one of the biggest Big Nos ever: [[spoiler: after defeating Frieza on a crumbling Namek, Goku tries to escape on Frieza's ship, but unfortunately, the ship was damaged and plummets into a fissure to dissolve in lava, leaving our hero with no way to escape.]]
** Goku uses instant transmission to do a point blank kamehameha on Cell [[http://www.youtube.com/watch?v=eg1bCXFLKdY here]].
* ''SayonaraZetsubouSensei'' subverts this by having one of the girls peep on their (male) teacher, and when they knock over the privacy wall and each side gets a good eye-full of the other, only he {{screams|LikeALittleGirl}}, much to his consternation.
* Chitose utters these quite often when having to deal with his self-proclaimed mothers on ''HappyLesson'', but the entire Biology class gets one when the lesson of the day is: "Dissection - Live chicks".
* Satoko plays this straightforward the anime-exclusive "Disaster Awakening" chapter of ''[[VisualNovel/HigurashiNoNakuKoroNi Higurashi No Naku Koro Ni Kai]]'' when she [[spoiler:sees the bodies of her schoolmates in the aftermath of a disastrous toxic gas leak that wiped out virtually the entire population of 2,000 people in her village other than herself]].
* In the first ''InuYasha'' movie (both the English and Japanese versions) Kagome lets out a rather chilling one after [[spoiler:being forced to shoot Inuyasha by the BigBad]] Complete with lack of BGM and echoes. It is used rather effectively though.
* In ''History's Strongest Disciple Kenichi'', during a financial crisis, Miu has the masters of Ryouzanpaku give lessons to small children. Kenichi is amused to see the straight-faced (but cruel) Akisame being run ragged by the hyperactive kiddies, but enacts this trope when he later sees what [[http://www.onemanga.com/Historys_Strongest_Disciple_Kenichi/191/12/ kind of effect his teachings have]].
* At the end of the first ep of ''NyanKoi'', Junpei lets out one in its full Engrish glory.
* In the manga version of ''{{Pretear}}'', Himeno lets out a Big No when she realizes that her carelessness has led to [[spoiler:Shin's life being drained away]].
* In ''{{K-On}}'', Mio lets out one of these after her on stage... accident. Involving a "[[PantyShot bowl of rice]]".
* The sixth volume of the ''Manga/KingOfThorn'' manga devotes a full page to a Big No [[spoiler:by Shizuku when Kasumi dies]].
* ''Anime/{{Pokemon}}''
** Dawn does one after [[spoiler:reliving her Pikari incident ''in the middle of a contest'']].
** Pyramid King Brandon also does it whenever he disapproves of something.
** Mars has one when [[spoiler:Cyrus throws himself into the new universe Dialga and Palkia have created just as it's starting to disappear.]]
** Nando [[TheStoic (?!)]] gets one when [[spoiler:Ash's Heracross knocks out his Kricketune with Megahorn.]]
* In ''TransformersArmada'', Optimus Prime lets out one when [[spoiler:Smokescreen]] is killed.
* ''Anime/SCryed'' has the main character, Kazuma, shouting this trope involving the death of a character.
* In episode 11 of ''GhostStories'' Satsuki yells this when her brother says he put a CreepyDoll in the trash can and she remembers when the doll called her to say it's at the trash can.
* ''VisualNovel/SchoolDays'':
** Sekai yells this in the last anime episode, [[spoiler: when Kotonoha and Makoto kiss in front of her.]]
** In episode 10 Nanami [[HeroicBSOD becomes extremely upset]] after the SchoolFestival, when [[spoiler: a sex tape of her and her boyfriend is shown to many classmates as a cruel joke from Otome's GirlPosse.]] She lets out one of these before collapsing to the ground in tears.
* In season 2, episode 6 of ''Manga/GirlsBravo'', a cross-dressing Yukinari screams this at one point.
* Melfina of ''OutlawStar'' screams "NO!" in episode 17 which stops Harry's [[MindRape hacking attempt]] on her.
* In chapter 52 of ''MiraiNikki'' Yukiteru does this when [[spoiler: Yuno asks him to kill her]].
* In the final scene of the last episode of ''{{Anime/Monster}}'' we are treated to a flashback of Nina's and Johann's childhood where their mother is [[spoiler: forced to give one of them up for an experiment.]] The two hold onto their mother's hand begging her not to let go, and their mother hesitates [[spoiler: before handing over Nina]] who proceeds to scream "No!" in horror as [[TearsOfFear tears]] spring from [[spoiler: her]] eyes.
* ''{{Gankutsuou}}'': Victoria Danglars does this in the 8th episode before fainting.
* ''HaloLegends: Specifically in ''The Duel'' Fal [[spoiler: discovers the dead body of his wife and lets out his "NOOOOOOOOO"]].
** His inflection makes it a bit difficult to tell, but it seems more like an inarticulate SkywardScream.
* Used in ''{{Karin}}'' (also called ''Chibi Vampire'') in conjunction with BigOMG when Winner sees his most recent test score. [[http://www.youtube.com/watch?v=6dJ-C-1Hrw0 Link]]
* In ''Manga/HidamariSketch'', Miyako seems to inspire a number of these.
* Golden Gash/Zatch Bell has [[http://www.youtube.com/watch?v=ylrtHz4qgO0#t=0m11s this]].
** Similarly, there's [[TransformingMecha Coral Q]], who responds to being hit by ''any'' attack with an enormous [[LargeHam "NOOOOOOOOOO!"]]
* Recently in ''Anime/YuGiOh5Ds'' there was Yusei's BRUNOOOOOOOOOOOOOOOOOOOOOOO!!!
** Actually, he has two of them. In the same episode.
* A majority of the regular cast in episode 10 in the ''Air Gear'' anime let out a huge "NOO!!!!" after Akito kisses [[EvenTheGuysWantHim Ikki]].
* ''RosarioPlusVampire'': Kurumu in chapter 41 of the second manga season. See it [[http://www.mangareader.net/rosario-vampire-ii/41/18 here]] and [[http://www.mangareader.net/rosario-vampire-ii/41/25 here]].
* In the first episode of ''Anime/FutureGPXCyberFormula ZERO'', Asuka lets one out when she sees Hayato lying down on the ground with his car completely destroyed after his car crashed down from the track with him in it at the English Grand Prix.
* In ''VisualNovel/{{Air}}'' when [[spoiler:[[TearJerker Misuzu dies,]] her mother figure]] shouts out her name in this fashion ([[spoiler:"MISUZUUUUUUUU!"]]).
* In ''KannazukiNoMiko,'' Himeko releases two when [[spoiler: Chikane rapes her]]. The English is [[http://www.youtube.com/watch?v=16FX8vgMurE&feature=related even better]].
* ''PrincessPrincess'': Sayaka in episode 7 after [[HoYay Yuujirou kisses Tooru]].
* ''Manga/AxisPowersHetalia'': Chibitalia when HRE tries to make him a part of the Holy Roman Empire.
* ''RomeoXJuliet'': Juliet when [[ItWasHisSled Romeo dies]].
* ''ReimeiNoArcana'': Nakaba wakes up screaming this when she has a dream foreseeing Caesar getting killed. She has another one when her hair is dyed black.
* ''Manga/DailyLivesOfHighSchoolBoys'': Hidenori had one in his InternalMonologue when [[WrongGenreSavvy the literature girl]] is sitting next to him again. Why? She's projecting the image of her novel's male protagonist onto him, which weirded him out.
* In ''TokyoMewMew'', Ichigo's boyfriend screams this when Ichigo is about to be kissed by Kisshu and Ichigo screams this as well before knocking Kisshu away.
* ''Manga/MagicKnightRayearth'': Princess Emeraude releases one when [[spoiler:Zagato dies in a battle with the Magic Knights.]]
* Happens in a couple of Hinako Takanaga's works:
** In ''Manga/TheTyrantFallsInLove'', the homophobic HotScientist Souichi does this whenever his [[SempaiKouhai lab assistant]] Morinaga comes onto him. Matters are confused because he doesn't want to face up to his potential homosexuality and so pretends to resist Morinaga to satisfy his own guilt even when he secretly wants to have sex - the BigNo comes out when he ''really'' doesn't want it and needs to make that clear.
** In ''Manga/LittleButterfly'' Kojima pushes his boyfriend Nakahara off with this whenever a makeout session gets too intense and it looks like [[CoitusEnsues sex might happen]]. Needless to say, this happens quite a lot.
* ''Manga/CardCaptorSakura'': Meiling lets out one when Syaoran is injured by The Shot, which she accidentally released.
* In ''Manga/{{Narutaru}}'' Hiro understandably lets these out as she is ''raped with a test tube''.
* In ''Anime/MawaruPenguindrum'' Ringo does this as a drugged up Tabuki chases her.
* In ''Manga/{{Naruto}}'' Izumo yells this in his mind [[spoiler: a few seconds before Hidan kills Asuma.]]
* During the traumatic end of the first episode of ''Manga/AttackOnTitan'', Eren Yeager lets loose with a big ''"YAMEROOOOOOOOOO!"'' [[note]]"STOP IT!"[[/note]] as [[spoiler:his mother is about to get eaten]]. In the manga, this scene is served with a proper BigNo.
* In ''Manga/YuYuHakusho'', when Kuwabara is struck down by Toguro, Yusuke lets one loose.
* In the first episode of ''Manga/GochuumonWaUsagiDesuKa'', Tippy does one when Cocoa is holding him too long. At first she's confused by it, but Chino tries to play it off as ventriloquism. However, Tippy is later revealed to be [[TalkingAnimal able to talk]].