* Dame Judi Dench as Queen Elizabeth. "But I know something of a woman in a man's position. Yes, by God, I do know about that."
** When Viola speaks up in defense of the theater, Wessex tries to get on Elizabeth's good side by disagreeing with her, claiming that he'd wager his entire fortune. Elizabeth coolly responds, "I thought you were here because you had none."
** Every second she is on screen. She didn't win an Academy Award for nothing.
--> "Have her then, but you're a lordly fool. She's been plucked since I saw her last and not by you. Takes a woman to know it."
* Burbage's little bit of crow-eating, and subsequent awesome, after Tilney closes the Rose:
-->'''Burbage:''' The Master of the Revels despises us all for vagrants and peddlers of bombast. But my father, James Burbage, had the first license to make a company of players from Her Majesty, and he drew from poets the literature of the age. We must show them that we are men of parts. Will Shakespeare has a play. I have a theatre. The Curtain is yours.
* Wabash amazing everyone by overcoming his stutter and [[StutterStop delivering the opening monologue with perfect diction]].
* Similarly, when Fennyman overcomes his stage fright and suddenly shows his acting chops and leads into the most heart-wrenching scene of the play.
* After Henslowe gets [[spoiler:Viola]] to go onstage as Juliet in place of Sam (and after he's spent the film trying to get Shakespeare to follow tradition):
-->'''BURBAGE:''' We'll all be put in the clink.
-->'''HENSLOWE:''' (casual shrug) See you in jail.