* Just as Mario & Luigi are cornered by Princess Shroob and several Shroob soldiers [[spoiler:after the former removes her Peach disguise on the mothership]], one of the Shroobs suddenly begins taking pictures before blasting the rest and their princess away with its blaster before revealing itself to be [[BigDamnHeroes Kylie Koopa in disguise]].
* The Mario Bros destroying the Shroob mothership.
* There's something eerily awesome about entering the Koopaseum to find ''an army'' of Shroobs reveling in their tyranny. If you didn't already realize how much power and control the Shroobs had on the situation, that was the moment it clicked.