* Tramp viciously attacking the rat [[PapaWolf to protect the baby]].
** Even more so, his brief conference with Lady. It really shows how close they are; they didn't even need to discuss the situation, they play off each other so well. Also, Tramp's tone when he comes bursting onto the scene; he's deadly serious, because he can ''tell'' that Lady is deadly serious. There's no ''time'' for jokes, and he doesn't make any. For Tramp, that's a big deal.
---> '''Tramp:''' What's wrong?\\
'''Lady:''' A rat. In the baby's room!\\
'''Tramp:''' [[LetsGetDangerous How do I get in?]]\\
'''Lady''': The little door on the porch!\\
(''Tramp sprints through the dog door to get inside the house'').
* Trusty regaining his sense of smell, successfully tracking the pound wagon carrying Tramp, and knocking it off the road!
* Trusty's always been the big, goofy friend, but he took a ''serious'' [[TookALevelInBadass level in badass]] when they realized Tramp was about to be killed for something he hadn't done.
--> '''Jock:''' What are we going to do?\\
'''Trusty:''' We'll run 'em down.\\
'''Jock:''' (''flustered'') Ay, and ''then''?\\
'''Trusty:''' (''darkly'') [[LetsGetDangerous We'll hold 'em. Hold 'em at bay...]]
** It's a reminder that Trusty's not just a retired hunting dog--he used to ''track criminals'' for a living. His owner is probably a retired policeman or something along those lines. Trusty ''knows'' what he's doing, weak sense of smell or none.
---> '''Trusty:''' Why, some of the finest people I ever tracked down were jailbirds!
** Going off of that, the absolutely ''bonechilling bay'' Trusty gives when he's caught the scent.
* When Lady's been taken to the pound and is visibly terrified of the rougher dogs staring at her and laughing about her collar, Peg, who's a ''Pekingese'', steps out of the woodwork and immediately shuts the much larger, much more dangerous dogs up; it's a small thing, but to ''Lady'' it clearly meant the world.
--> "Hey! Hey! Hey! Lighten up, will ya?! Can't you see the poor thing's scared enough already?!"
** Bill the pound worker petting Lady and reassuring her with a very telling "All right, baby, they've come to take you home. You're too nice a girl to be in this place." The man's job might require him to do some unfortunate things, but he clearly loves dogs.
* Tramp saving Lady from a ''pack'' of feral dogs each at least twice his size.
** What's more, they ''freeze'' the minute they see him.
* Lady calling Tramp out for sleeping around---sure, audiences of today may find it rather slow and sappy, but it really knocks sense into him and makes him realize he's been a jerk.
* It's not character based, but the animation in the scene where Darling is rocking the cradle is stunning. Her skirt is swaying, the cradle is rocking, the bow on the cradle is moving slightly behind the cradle movement, the curtains are swaying in the breeze, the trees are moving their branches in coordination with the breeze, all at the same time. Perfectly coordinated, and done in hand drawn cell animation in 1955. Today's computer generated animation, even with masterful physics engines, don't even come close to the beauty of this scene.
* At the end of the pound scene when Bill is coming to get Lady, look at the hole just before [[TunnelKing Dachsie]] covers it up. There's a light at the end of the tunnel. He really did tunnel them out!
* Despite how gentle a dog Lady is, she ''will'' viciously bare her fangs and attempt to attack whenever big, ugly rats gets a little too close to her owners' home.