* Sweet Tooth's EstablishingCharacterMoment, terrifying as it is. He barges into the RoguesGallery meeting, kills Penguin's bird Chilly Willy, and says he's their leader now. Everyone quickly defers.
* Every time Sweet Tooth makes a candy pun, he actually pulls out the candy the pun is based on from somewhere on his person. As if stage acting wasn't hard enough, and yet Jeff Blim doesn't miss a beat.
* Credit to Robin; when Sweet Tooth captures him, using Baby Candy in a WoundedGazelleGambit, he shows no fear despite the fact that Sweet Tooth is threatening to kill him at dawn. Instead, he glares at the man and prepares to FaceDeathWithDignity.
* ''To Be a Man''. Superman fighting Batman. In song. It's as glorious as it sounds.
* Say it with me, folks: "[[ComicBook/AllStarBatmanAndRobinTheBoyWonder I'm the God. Damn. BATMAN!]]"
** Especially notable is that they take the most infamously {{Narm}}y line in Batman history and make it genuinely badass.
* Robin of all people grabs the SmartBall and becomes the conscience. After Batman saves him, Robin tells him they can't leave the citizens of Gotham to perish. Batman insists that Sweet Tooth said everyone voted for Robin to die [[PapaWolf and he can't stand for that]]. Robin gets irate, pulls up the poll on a smartphone, and shows Batman that everyone voted to ''save'' Robin.
* For that matter, when we hear why the Gotham citizens chose to sacrifice their lives rather than let Sweet Tooth kill Robin; they may not like the hero, but he's a child. [[EveryoneHasStandards Children are off-limits]]. Besides, Batman likes Robin so he can't be all bad.
* Batman's speech to Superman about why superheroes are awesome is enough to make any fan of classic comic books cry.
* At the finale, [[spoiler: Superman flies around the world to turn back time (both a BrickJoke and a shout-out to [[Film/SupermanTheMovie one of his movies]]), and then hurls Sweet Tooth's warheads into the sun, after Batman called him to apologise and ask for help.]]
** "And ''that'' is why they call me '''Super'''man!"
* [[spoiler: When the entire Justice League (and Spider-Man) show up during the final song! Also doubles as SugarWiki/AwesomeMusic when they sing in harmony.]]