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* In a very real way, the entire movie can be seen as one for [[Franchise/UniversalHorror the classic Universal monsters]]. When [[Film/Dracula1931 Count Dracula]], [[Film/Frankenstein1931 the Frankenstein Monster]], and [[Film/TheWolfMan1941 Larry Talbot]] went out, they did it with style.
* At one point when they've escaped {{Dracula}}'s castle, the vampire goes after Lou to bring him back with his powers of MindControl. Lou actually holds out for a few moments before giving in and skipping merrily to his (intended) doom. Hey, it's not much, but how many people ever beat [[BigBad Dracula's]] powers of control, even temporarily?
* Doubling as two [[SugarWiki/FunnyMoments Crowning Comic Moments]], when being chased through the castle by [[FrankensteinsMonster the Monster]], Lou jerks a black tablecloth off a table, leaving the lights standing. He does a double take before [[BreakingTheFourthWall boastfully showing it to the audience]] and retreating behind a door. The Monster opens it and Lou comes out, using the tablecloth to make like Dracula:
-->'''Lou:''' Back... back...
-->'''The Monster:''' Yes, Master.
** At which point Lou drops the tablecloth and smugly tells Bud, "He thinks I'm Dracula!"
* Dracula, running from the Wolf Man, turns into a bat and flies off a balcony. Wolf Man leaps, grabs the bat in both hands, and [[DisneyVillainDeath they both fall into the sea to their apparent deaths]].
* For an Abbott and Costello film, this had a violent ending. And although the monsters seem to meet their demise, PopCultureOsmosis had kicked in by this time and audiences figured that [[BackFromTheDead they won't stay down for long]] (however, this ultimately ended up being the final film to feature these versions of the characters, so whether any of them did survive remained unknown). However, there was one human death (Sandra), a rarity for Abbott and Costello adventures, even when it's a villain.
** The source of that death: Frankenstein turning on the mad Sandra when she tries to control him, might also count.
* When the {{Jerkass}} [=MacDougal=] and the sheriff try to arrest Wilbur and Chick in the middle of the final chase Wilbur has a great comeback that makes the two men flee when they see Frankenstein.
--> '''Wilbur:''' You still want your exhibits?
--> '''[[=MacDougal=]]:''' Of course I do.
--> '''Wilbur:''' Well here comes one of them now.
* Chick being willing to go to the island to rescue Wilbur and Joan after realizing the monsters are real.
* Stevens and Joan taking out Frankenstein's monster by setting the pier on fire.
* The Monster walking down a flaming dock as it bursts into flames is an iconic image and bookends the character's original "death" in ''Film/Frankenstein1931''.
* The last-minute "[[VoiceOnlyCameo appearance]]" of Creator/VincentPrice as the Invisible Man.

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