* Anytime the Rune is said.
* At the end of the book, Charles Wallace states that [[spoiler:an elderly Beezie]] used the rune to place ''[[spoiler:herself]]'' between the main characters and all the powers of darkness.
* While trapped inside an injured Chuck, Charles Wallace is visited by a unicorn who tells him that he needs to leave Chuck behind to regroup and figure out a new plan. [[spoiler:Charles Wallace finally completes his character arc, masters his pride, and has faith in Chuck to carry on, defeating the Echthroi's trap and allowing him to continue an important step in his journey.]]