* Doctor Tomorrowland, currently a member of the Disney-sponsored hero team Imagination in the ''GlobalGuardiansPBEMUniverse'', started out as part of the team's background extras. She rose to the rank of AscendedExtra, and then supporting character, and then to featured player, all because one of the players decided to start including her more nad more in the story. She later became a full-blown player character.
* One example is ''EvangelionR'', in which Maya Ibuki goes from a [[BridgeBunnies Bridge Bunny]] to a major character.
* [[SonicTheHedgehog Dr. Robotnik]] (in his ''AdventuresOfSonicTheHedgehog'' incarnation) has gone on to become one of the most popular WebAnimation/YouTubePoop characters, spawning hundreds of videos. This also happens with other characters from other sources, though LargeHam villains seem to get this the most.
** I.M. Meen stars in about 4 minutes of {{cutscene}}s and is somehow nearly as big as the CD-I ''Zelda'' games, despite them having at least five times more cutscenes than I. M. Meen does.
** Another notable AscendedExtra in the WebAnimation/YouTubePoop universe is Morshu the Shopkeeper, who is one of the most used characters despite having only ''two lines'' in the entire game of ''[[VideoGame/TheLegendOfZeldaCdiGames Link: The Faces of Evil]]''
* Similarly, Drew Pickles from ''{{Rugrats}}'' has become the icon of the WebVideo/BarneyBunch, thanks to an AlternateCharacterInterpretation PlayedForLaughs (specifically, a closet semi-StraightGay).
* In ''DarwinsSoldiers'', Dr. Zanasiu's assistant originally didn't even have a name, and was planned to get killed off rather quickly. He didn't, however, and after earning a name (Zachary) he soon became one of the most important characters in the series.
* There is a Youtube user called [[http://www.youtube.com/user/WTLNetwork WTLNetwork]] who makes WebAnimation/{{YouTube Poop}}s of ''ThomasTheTankEngine''. A lot of his poops involve the one-shot character of the Fat Controller's Butler (originally a OneSceneWonder) as a full-blown character and the Fat Controller's lover!
* Starting to be a regular thing in the ''WhateleyUniverse''. One of the campus jokes, the kid with the spirit of the squirrel, got a role in the ''Phase'' novels, and then her own story. Side character and campus nuisance Greasy is getting a Christmas special. BigBad Don Sebastiano got a point-of-view story, and has gotten more screen time. And on and on...
* Shinji Ryougi, of ''YukariIsFree'', originally started as a one-shot gag character to insult a Supporting Character, simply being addressed as Gang Member 122. By the next season, he had elevated to a full-blown member of the Main Cast and actually being referred to by name. The same has happened to other characters, most notably Rei Kakizaki, Kasumi Chiba, and Elesia Rumiko, all of whom started out as extras and were elevated to Supporting Roles and given unique subplots.
* Happens all too often in ''SurvivalOfTheFittest'', when characters thought only to be minor characters manage to survive to the end thanks to sheer luck, or simply becoming well-noticed. Two examples:
** [[{{Meganekko}} Lulu Altaire]] was originally just a member of a four-person posse including [[CloudCuckoolander Anna Kateridge]], [[TeamMom Amanda Redder]], and (regrettably) [[TheScrappy Liam Black]]. Three in-game days later, the death of everyone else she'd met, Lulu becomes one of the ''final characters'' alive on the island. [[spoiler:She ultimately places second overall in the season, after losing the final battle with J.R. Rizzolo.]]
** [[DeadpanSnarker Aston]] [[{{Asexual}} Bennett]] was originally only a supporting member of another rag-tag group of misfits. Then, after the group is unceremoniously split up when one of the members turns on the group and is accidentally killed, and the boy she ''[[UnresolvedSexualTension may]]'' have had a crush on is murdered right in front of her, she steps up to the plate and sets off on a [[ItsPersonal revenge quest]] against his killer.
* In ''TheGunganCouncil'', [=LiSah=] Mao and Deagan Hunt were introduced as [[NonPlayerCharacter NPC's]] but are now their own [[PlayerCharacter player characters]].
* In the ''Fanfic/NineteenEightyThreeDoomsdayStories,'' a BroadStrokes AU of ''Literature/NineteenEightyThreeDoomsday,'' there's Sopron. Originally just a border area in the source material, she's become a veritable Survivor-Nation in her own right [[spoiler: as well as being [[Webcomic/AxisPowersHetalia Austria and Hungary's daughter]]]].
* Pretty much the entirety of the cast of ''WebVideo/{{Arenas}}'', which in the Quake and Doom universes were just selectable characters for the players.
* ''WebAnimation/{{RWBY}}'': British bunny girl Velvet appeared for one short scene in Episode 11, where [[TheBully Cardin]] is shown painfully tugging on her ears to demonstrate the setting's FantasticRacism against Faunas, and [[KickTheDog what a jerk Cardin is]]. She very quickly became an [[EnsembleDarkhorse unexpected fandom favourite]] and Montyoum was so impressed by the fandom's reaction to her that he has promised a larger role for her in Season 2. From previews, it appears that she's even getting her own team.