* ''WebAnimation/TheNekciMenijShow'' has all of its female characters jockeying around each other for this position in the music world. Beyonce is the most obvious example of an Alpha Bitch, based on her ostentatious behavior and her need to command every room she's in.
* ''WebAnimation/TheMostPopularGirlsInSchool'' is, as the title suggests, about a whole ''bunch'' of Alpha Bitches competing for supremacy. This show is basically ''Alpha Bitch: The Web Show''. Mackenzie Zales, who is [[MemeticMutation head cheerleader, homecoming queen, and a part-time model]], her arch-rival, socialite Shay van Buren (AlphaBitch-itude seems to run in the van Buren family, as Shay's mother and older sister Cameron are former ones, and younger sister Mikayla is the AlphaBitch of her elementary school.), and Tanya Berkowitz are all the Alpha Bitches of the Overland Park Cheer Squad, the van Buren family, and the Atchison Cheer Squad respectively. Probably the biggest bitch of the first group, though, is [[HairTriggerTemper Brittnay Matthews]], a cheerleader who is TheStarscream to Mackenzie.
* ''WebAnimation/TeenGirlSquad'', being a parody of high school stereotypes, naturally features one, [[TheCheerleader appropriately nicknamed Cheerleader]]. Who, as we eventually learn, isn't even a real cheerleader. She's also nowhere near as popular as this trope usually entails.
* ''WebAnimation/{{RWBY}}'': Weiss Schnee is a rare protagonist example. At least initially. She [[CharacterDevelopment gets]] [[TookALevelInKindness better]]... [[JerkWithAHeartOfGold sorta]]. To the point of being a LovableAlphaBitch, at best.
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