Male characters, especially cocky ones, generally don't like being beaten by a female character.
Related to Samus Is a Girl
. Might need a better title.
- At the end of the Doctor Who episode Utopia, the Master utters his anger at having been fatally wounded by Chantho. He's killed in a similar fashion by Lucy in Last of the Time Lords and jokes that women often do that.
- In Advance Wars, Eagle expresses his surprise when Sami, who has just beaten his whole army, turns out to be female.